PTI / HORIBA Scientific

PlateReader_400pxAbout FluoDia T70 High Temperature Microplate Reader

The FluoDia T70 high temperature fluorescent plate reader is manufactured by Otsuka Electronics Co., Ltd, a Japanese scientific instrumentation company who made numerous plate readers for various companies on OEM basis. It was developed jointly by Otsuka Electronics and Photon Technology International and marketed by the later.

The acquisition of Photon TechnologA-P3196-20140401-01y International by Horiba Scientific Instruments offers a unique opportunity for customers to own this top performance plate reader at a fraction of its original price—a bargain that nobody should miss!

Key Features:

FluoDia T70 is a filter-based, high temperature microplate reader for measuring fluorescence intensity of samples in 6 to 384-well microplates.  The most outstanding features of FluoDia T70 are: reproducibility, sensitivity, temperature control, and dynamic range.

  • Highest Reproducibility: This is the ability to give repeatedly the same results, i.e. no inaccuracy due to equipment. The highest reproducibility of FluoDia T70 makes it possible to monitor minor changes in a signal that would otherwise be considered as the standard deviation of the machine.
  • Highest Sensitivity: FluoDia T70 is one of the most sensitive fluorescence plate readers on the market. It can read as low as 2.4 fmol/well of sodium fluorescein.
  • Temperature Control: The exclusive heating stage allows Fluodia T70 to control   temperature up to 75° C with accuracy of ±0.3°C and homogeneity of ±0.3°C across the plate. This allows it to be used for a wide range of applications, such as Protein Thermal Shift Assay, RNA Invader® assay, ThermoFluor® binding assay, denature of a wide range of proteins, in vitro fibrillogenesis, et al.
  • Widest Dynamic Range: This defines the range of measurements over which the machine is capable of measuring. FluoDia T70 can detect signals within 7 orders of magnitude, which is the widest dynamic range of all plate readers on the market. This allows it to measure both very strong and very weak samples in the same plate.
  • FluoDia T70 has many other important features:
    • Dual wavelength measurement capability allows it to be used for applications such as FRET, intracellular ion or pH et al.
    • Multiple points/well measurement feature allows users to choose which points in a given well signals should be collected when using 6, 12, 24 and 48 well plates.
    • Linear or two dimension butterfly sample mixing, with user defined velocity and duration guarantees proper sample mixing before and during mulit-cycle measurements.
    • Quantitative measurements
    • Software complies with FDA21 CFR part11, which is useful for biotech and pharmaceutical companies
    • Barcode Reading Capability
  • Pricing: There is no better time to buy FluoDia T70 than now—we are running a promotion with  huge discount!! Ask for a quote—you will be surprised by how much you can save!!!
Light source                             Quartz Halogen (I2) lamp,: 2000 Hours  (320–830 nm)
Light guide                             Quartz fiber (1.4 mm) & condenser lens
Optics                                       Top-to-top
Wavelength Selection         Interference filters
Filter ports              4 each for exc/emi. Automatic software controlled
Detector                                  Side-on type PMT  (185–830 nm)
Measurement method           Photon counting
Integration time                       10 msec–10sec
Number of averages               1–100 times/well
Stage                                       X – Y stage with automatic sensing of the well-center
Stage Calibration                     Automatic calibration using the calibration plate
Sample mixing                        Mixing form, time, velocity all software controlled
Temperature control                 Exclusive heating stage
Heating stage                       Flat heater plate, 96-well or 384-well heater plate
Dual P.I.D. control. Built-in (123°C) thermal fuse   
Plate type         6-384 well plate, strip tubes (when using 96-well heating stage). Transparent, black, white
Bar-code reader:                   Keyboard Wedge Output (up to 16 characters)
PC requirements:                    Windows OS: Windows NT 4.0, 2000 , XP, Windows 7
External communication        RS-232C or USB
Power supply   120 V/230 V/100 V, 50/60 Hz
Dimensions (W x D x L) 380 x 505 x 200 mm
Weight Approx. 20 kg

Specifications are subject to change without notification

Sensitivity           2.4 fmol/well fluorescein; 4-MUF: 0.1 pmol/well
Reproducibility                       CV ≤ 1.5%
Dynamic range                         0 –16,777,215 counts
Linearity                                   Correlation coefficiency ≥ 0.995
Dark level                                 ≤ 3 x 10-4 % full scale
Stability                                    ≤ 3 x 10-4 % full scale/8 hours
Measurement time Single wavelength       ~ 40 sec/96 wells , ~ 110 sec/384 wells

Dual wavelength         ~  51 sec/96 wells (if measured per well) ~ 67 sec/96 wells (if measured per plate) 

Temperature Control: Range                          RT +5°C to 75°C

Mode of Heating        Temperature stepping, one or two points incubation

Accuracy                     ± 0.3°C

Homogeneity              ± 0.3°C

Temp. Rise time (Heating Stage)        ≤ 6 min. (up to 75°C from R.T.)

Rise time (Sample, from 20°C to 68°C): ≤ 5 min. without pre-heating, ≤ 3 min. if pre-heated at 45°C, ≤ 1 min. if pre-heated at 68°C

Plate Dimensions Width                                   86.0 ± 0.8 mm

Length                                  128.0 ± 0.8 mm

Height                                  Without use of heater plate: 25 mm

Without use of heater plate : ~ 20 mm

FluoDia T70 can be used to measure the intensity of fluorescence samples in the UV/VIS range (340-820 nm), whether it is DNA, RNA, Protein, live cells, blood samples or nano-particles.

  • Temperature controlled measurements:
    • Protein Thermal-shift Assay  (Download Thermal Shift Assay Protocol)
    • RNA Invader assay ( Invader® is a registered trademark of Third Wave Technologies Inc.)
    • ThermoFluor Binding assay. (ThermoFluor® is a registered trademark of 3-Dimensional Pharmaceuticals, Inc.)
    • Hydrophobic interaction upon unfolding
    • Monitor fibrillogenesis in vitro
  • Quantitative DNA measurement
    • dsDNA
    • ssDNA
    • PCR product
  • Quantitative RNA measurement
    • mRNA
    • RNA Invader assay
    • tRNA
    • rRNA
    • siRNA
    • Total RNA
    • PicoGreen RNA
    • RiboGreen RNA
  • Protein
    • Quantitative protein measurement
    • Protein Quantification
    • Fluorescence ELISA
  • Detect enzyme activity of proteins
    • Protease
    • Tyrosine Kinase
    • Matrix metalloproteinases (MMP)
    • Elastase
    • L-asparaginase
    • Phosphatase 2A
    • b-galactosidase
    • GFP-based assay
  • Cell based assay
    • Live/Dead/Viability/cytotoxicity
    • Factors affecting cell viability.
    • Proliferation
    • Examining factors affecting cell proliferation in a large number of samples.
    • Multi-drug resistance assay
    • Resistance of tumor cells to anticancer drugs.
    • Phagocytosis
    • Factors affecting phagocytosis, phagocytotic deficiencies diagnosis.
    • Adhesion assay
    • Factors affecting cell adhesion, including leukocyte, neutrophil, monocyte.
    • Respiratory Burst
    • Reactive oxygen species, H2O2 .
  • Blood sample based assay
    • Cholesterol (also for food samples)
    • Glucose
    • Glucose oxidase
    • Glutamic acid, glutamate
    • Monoamine oxidase
    • Cellulase
    • Acetylcholine and acetylcholinesterase
    • Phosphatidylcholine-specific phospholipase C, D sphingomyelinase
    • Respiratory burst in patient leukocytes
    • Lipid peroxidation

Exchangeable Heating Stage:

  • H-1: Flat heating stage with a temperature limit of 75 °C. It can accommodate 6, 12, 24, 48, 96, 384-well
  • H-2: 96-well heating stage with a temperature limit of 75 °C.  It is designed for U- or V-shaped 96-well microplates as well as strip tubes.
  • H-3: 384-well heating stage with a temperature limit of 75 °C.  It is designed for U- or V-shaped 384 well microplates.

Filters have a 20nm bandpass (±10nm) unless otherwise specified

  • Standard filters:  480 DF20 (Excitation) / 530DF20 (Emission)
  • Optional Filters:  340, 365,390, 410, 410, 425, 450 *, 465, 486, 500, 500, 515, 530*, 560, 560 *, 565, 570, 580, 590, 620, 645, 665, 680

*filters of 10nm bandpass

Replacement Lamp

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  • Serrano, I., Pelliccione, S. and Olmedilla, A. (2010) Programmed-cell-death hallmarks in incompatible pollen and papillar stigma cells of Olea europaea L. under free pollination. Plant Cell Rep, 29, 561-572.
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  • Abbott labs
  • Amgen Inc.
  • Astex Technology Ltd
  • Bayer
  • Boehringer Ingelheim Pharma GmbH & Co.
  • Evotec
  • Gen-Probe Incorporated
  • Glaxo SmithKline
  • Hoffmann-La Roche Inc.
  • Merck & Co., Inc.
  • Millipore Corporation
  • MLT Research Ltd
  • Novartis
  • Pfizer Global research & Development
  • Sanofi Aventis
  • Schering-Plough
  • Sensor technologies Inc.
  • USB Corporation
  • 3M
  • Duke University
  • National Institutes of Health (NIH / NIEHS)
  • National Research Council of Canada
  • Sandia National Laboratories
  • Southern Illinois University
  • Northwestern University
  • Oxford University
  • Trinity University
  • University of California Berkeley
  • University of California San Diego
  • University of Georgia
  • University of Illinois at Chicago
  • University of Texas
  • University of Toronto
  • Vanderbilt University
  • Yale University
  • Toronto General Hospital
  • V. A. Medical Center
  • And many more…