About FluoDia T70 High Temperature Microplate Reader
The FluoDia T70 high temperature fluorescent plate reader is manufactured by Otsuka Electronics Co., Ltd, a Japanese scientific instrumentation company who made numerous plate readers for various companies on OEM basis. It was developed jointly by Otsuka Electronics and Photon Technology International and marketed by the later.
The acquisition of Photon Technology International by HORIBA Scientific Instruments offers a unique opportunity for customers to own this top performance plate reader at a fraction of its original price—a bargain that nobody should miss!
FluoDia T70 is a filter-based, high temperature microplate reader for measuring fluorescence intensity of samples in 6 to 384-well microplates. The most outstanding features of FluoDia T70 are: reproducibility, sensitivity, temperature control, and dynamic range.
- Highest Reproducibility: This is the ability to give repeatedly the same results, i.e. no inaccuracy due to equipment. The highest reproducibility of FluoDia T70 makes it possible to monitor minor changes in a signal that would otherwise be considered as the standard deviation of the machine.
- Highest Sensitivity: FluoDia T70 is one of the most sensitive fluorescence plate readers on the market. It can read as low as 2.4 fmol/well of sodium fluorescein.
- Temperature Control: The exclusive heating stage allows Fluodia T70 to control temperature up to 75° C with accuracy of ±0.3°C and homogeneity of ±0.3°C across the plate. This allows it to be used for a wide range of applications, such as Protein Thermal Shift Assay, RNA Invader® assay, ThermoFluor® binding assay, denature of a wide range of proteins, in vitro fibrillogenesis, et al.
- Widest Dynamic Range: This defines the range of measurements over which the machine is capable of measuring. FluoDia T70 can detect signals within 7 orders of magnitude, which is the widest dynamic range of all plate readers on the market. This allows it to measure both very strong and very weak samples in the same plate.
- FluoDia T70 has many other important features:
- Dual wavelength measurement capability allows it to be used for applications such as FRET, intracellular ion or pH et al.
- Multiple points/well measurement feature allows users to choose which points in a given well signals should be collected when using 6, 12, 24 and 48 well plates.
- Linear or two dimension butterfly sample mixing, with user defined velocity and duration guarantees proper sample mixing before and during mulit-cycle measurements.
- Quantitative measurements
- Software complies with FDA21 CFR part11, which is useful for biotech and pharmaceutical companies
- Barcode Reading Capability
- Pricing: There is no better time to buy FluoDia T70 than now—we are running a promotion with huge discount!! Ask for a quote—you will be surprised by how much you can save!!!
|Light Source||Quartz Halogen (I2) lamp,: 2000 Hours (320–830 nm)|
|Light Guide||Quartz fiber (1.4 mm) & condenser lens|
|Wavelength Selection||Interference filters|
|Filter Ports||4 each for exc/emi. Automatic software controlled|
|Detector||Side-on type PMT (185–830 nm)|
|Measurement Method||Photon counting|
|Integration Time||10 msec–10sec|
|Number of Averages||1–100 times/well|
|Stage||X – Y stage with automatic sensing of the well-center|
|Stage Calibration||Automatic calibration using the calibration plate|
|Sample Mixing||Mixing form, time, velocity all software controlled|
|Temperature Control||Exclusive heating stage|
|Heating Stage||Flat heater plate, 96-well or 384-well heater plate Dual P.I.D. control. Built-in (123°C) thermal fuse|
|Plate Type||6-384 well plate, strip tubes (when using 96-well heating stage). Transparent, black, white|
|Bar-code Reader:||Keyboard Wedge Output (up to 16 characters)|
|PC Requirements:||Windows OS: Windows NT 4.0, 2000 , XP, Windows 7|
|External Communication||RS-232C or USB|
|Power supply||120 V/230 V/100 V, 50/60 Hz|
|Dimensions (W x D x L)||380 x 505 x 200 mm|
|Weight||Approx. 20 kg|
Specifications are subject to change without notification
|Sensitivity||2.4 fmol/well fluorescein; 4-MUF: 0.1 pmol/well|
|Reproducibility||0 –16,777,215 counts|
|Dynamic Range||Correlation coefficiency ≥ 0.995|
|Linearity||≤ 3 x 10-4 % full scale|
|Dark level||≤ 3 x 10-4 % full scale/8 hours|
|Stability||Single wavelength ~ 40 sec/96 wells , ~ 110 sec/384 wells Dual wavelength ~ 51 sec/96 wells (if measured per well) ~ 67 sec/96 wells (if measured per plate)|
|Temperature Control||Range RT +5°C to 75°C Mode of Heating Temperature stepping, one or two points incubation Accuracy ± 0.3°C Homogeneity ± 0.3°C Temp. Rise time (Heating Stage) ≤ 6 min. (up to 75°C from R.T.) Rise time (Sample, from 20°C to 68°C): ≤ 5 min. without pre-heating, ≤ 3 min. if pre-heated at 45°C, ≤ 1 min. if pre-heated at 68°C|
|Plate Dimensions||Width 86.0 ± 0.8 mm Length 128.0 ± 0.8 mm Height Without use of heater plate: 25 mm Without use of heater plate : ~ 20 mm|
FluoDia T70 can be used to measure the intensity of fluorescence samples in the UV/VIS range (340-820 nm), whether it is DNA, RNA, Protein, live cells, blood samples or nano-particles.
- Temperature controlled measurements:
- Protein Thermal-shift Assay (Download Thermal Shift Assay Protocol)
- RNA Invader assay ( Invader® is a registered trademark of Third Wave Technologies Inc.)
- ThermoFluor Binding assay. (ThermoFluor® is a registered trademark of 3-Dimensional Pharmaceuticals, Inc.)
- Hydrophobic interaction upon unfolding
- Monitor fibrillogenesis in vitro
- Quantitative DNA measurement
- PCR product
- Quantitative RNA measurement
- RNA Invader assay
- Total RNA
- PicoGreen RNA
- RiboGreen RNA
- Quantitative protein measurement
- Protein Quantification
- Fluorescence ELISA
- Detect enzyme activity of proteins
- Tyrosine Kinase
- Matrix metalloproteinases (MMP)
- Phosphatase 2A
- GFP-based assay
- Cell based assay
- Factors affecting cell viability.
- Examining factors affecting cell proliferation in a large number of samples.
- Multi-drug resistance assay
- Resistance of tumor cells to anticancer drugs.
- Factors affecting phagocytosis, phagocytotic deficiencies diagnosis.
- Adhesion assay
- Factors affecting cell adhesion, including leukocyte, neutrophil, monocyte.
- Respiratory Burst
- Reactive oxygen species, H2O2 .
- Blood sample based assay
- Cholesterol (also for food samples)
- Glucose oxidase
- Glutamic acid, glutamate
- Monoamine oxidase
- Acetylcholine and acetylcholinesterase
- Phosphatidylcholine-specific phospholipase C, D sphingomyelinase
- Respiratory burst in patient leukocytes
- Lipid peroxidation
Exchangeable Heating Stage:
- H-1: Flat heating stage with a temperature limit of 75 °C. It can accommodate 6, 12, 24, 48, 96, 384-well
- H-2: 96-well heating stage with a temperature limit of 75 °C. It is designed for U- or V-shaped 96-well microplates as well as strip tubes.
- H-3: 384-well heating stage with a temperature limit of 75 °C. It is designed for U- or V-shaped 384 well microplates.
Filters have a 20nm bandpass (±10nm) unless otherwise specified
- Standard filters: 480 DF20 (Excitation) / 530DF20 (Emission)
- Optional Filters: 340, 365,390, 410, 410, 425, 450 *, 465, 486, 500, 500, 515, 530*, 560, 560 *, 565, 570, 580, 590, 620, 645, 665, 680
*filters of 10nm bandpass
- Kumar, S., Birol, M., Schlamadinger, D.E., Wojcik, S.P., Rhoades, E. and Miranker, A.D. (2016) Foldamer-mediated manipulation of a pre-amyloid toxin. Nat Commun, 7, 11412.
- Slack, R.L., Spiriti, J., Ahn, J., Parniak, M.A., Zuckerman, D.M. and Ishima, R. (2015) Structural integrity of the ribonuclease H domain in HIV-1 reverse transcriptase. Proteins: Structure, Function, and Bioinformatics, 83, 1526-1538.
- Rubio, M.A., Schlamadinger, D.E., White, E.M. and Miranker, A.D. (2015) Peptide Amyloid Surface Display. Biochemistry, 54, 987-993.
- Partskhaladze, T., Taylor, A., Lomidze, L., Gvarjaladze, D. and Kankia, B. (2015) Exponential quadruplex priming amplification for DNA-based isothermal diagnostics. Biopolymers, 103, 88-95.
- Nath, A., Schlamadinger, D.E., Rhoades, E. and Miranker, A.D. (2015) Structure-Based Small Molecule Modulation of a Pre-Amyloid State: Pharmacological Enhancement of IAPP Membrane-Binding and Toxicity. Biochemistry, 54, 3555-3564.
- Kumar, S., Schlamadinger, D.E., Brown, M.A., Dunn, J.M., Mercado, B., Hebda, J.A., Saraogi, I., Rhoades, E., Hamilton, A.D. and Miranker, A.D. (2015) Islet Amyloid-Induced Cell Death and Bilayer Integrity Loss Share a Molecular Origin Targetable with Oligopyridylamide-Based Î±-Helical Mimetics. Chemistry & Biology, 22, 369-378.
- Almeida, B., Abreu, I.A., Matos, C.A., Fraga, J.S., Fernandes, S., Macedo, M.G., GutiÃ©rrez-Gallego, R., Pereira, P.J.B., Carvalho, A.L.s. and Macedo-Ribeiro, S. (2015) SUMOylation of the brain-predominant Ataxin-3 isoform modulates its interaction with p97. Biochimica et Biophysica Acta (BBA) – Molecular Basis of Disease, 1852, 1950-1959.
- Serra-Vidal, B., Pujadas, L., Rossi, D., Soriano, E., Madurga, S. and Carulla, N.l. (2014) Hydrogen/Deuterium Exchange-Protected Oligomers Populated during AÎ² Fibril Formation Correlate with Neuronal Cell Death. ACS Chemical Biology, 9, 2678-2685.
- Schlamadinger, D.E. and Miranker, A.D. (2014) Fiber-Dependent and -Independent Toxicity of Islet Amyloid Polypeptide. Biophysical Journal, 107, 2559-2566.
- Kumar, S., Brown, M.A., Nath, A. and Miranker, A.D. (2014) Folded Small Molecule Manipulation of Islet Amyloid Polypeptide. Chemistry & Biology, 21, 775-781.
- Hebda, J.A., Magzoub, M. and Miranker, A.D. (2014) Small molecule screening in context: Lipid-catalyzed amyloid formation. Protein Science, 23, 1341-1348.
- Gogichaishvili, S., Lomidze, L. and Kankia, B. (2014) Quadruplex priming amplification combined with nicking enzyme for diagnostics. Analytical Biochemistry, 466, 44-48.
- Alhasan, A.H., Patel, P.C., Choi, C.H.J. and Mirkin, C.A. (2014) Exosome Encased Spherical Nucleic Acid Gold Nanoparticle Conjugates as Potent MicroRNA Regulation Agents. Small, 10, 186-192.
- Galano, J.J., AlÃas, M., PÃ©rez, R., VelÃ¡zquez-Campoy, A., Hoffman, P.S. and Sancho, J. (2013) Improved Flavodoxin Inhibitors with Potential Therapeutic Effects against Helicobacter pylori Infection. J. Med. Chem., 56, 6248-6258.
- Boivin, S., Kozak, S. and Meijers, R. (2013) Optimization of protein purification and characterization using Thermofluor screens. Protein Expression and Purification, 91, 192-206.
- Sandoval, K.E., Farr, S.A., Banks, W.A., Crider, A.M., Morley, J.E. and Witt, K.A. (2012) Somatostatin receptor subtype-4 agonist NNC 26-9100 decreases extracellular and intracellular A1-42 trimers. European Journal of Pharmacology, 683, 116-124.
- McCalla, S.E., Ong, C., Sarma, A., Opal, S.M., Artenstein, A.W. and Tripathi, A. (2012) A Simple Method for Amplifying RNA Targets (SMART). The Journal of Molecular Diagnostics, 14, 328-335.
- Luthi, A.J., Zhang, H., Kim, D., Giljohann, D.A., Mirkin, C.A. and Thaxton, C.S. (2012) Tailoring of Biomimetic High-Density Lipoprotein Nanostructures Changes Cholesterol Binding and Efflux. ACS Nano, 6, 276-285.
- Fujikura, J., Nakao, K., Sone, M., Noguchi, M., Mori, E., Naito, M., Taura, D., Harada-Shiba, M., Kishimoto, I., Watanabe, A., Asaka, I. and Hosoda, K. (2012) Induced pluripotent stem cells generated from diabetic patients with mitochondrial DNA A3243G mutation. Diabetologia, 55, 1689-1698.
- Tadokoro, K., Ishikawa, M., Suzuki, M., Saito, T., Suzuki, Y., Yamaguchi, T. and Yagasaki, F. (2011) Comparative quantitative analysis of BCR-ABL transcripts with the T315I mutant clone by polymerase chain reaction (PCR)-Invader method. Translational Research, 158, 169-179.
- Prigodich, A.E., Alhasan, A.H. and Mirkin, C.A. (2011) Selective Enhancement of Nucleases by Polyvalent DNA-Functionalized Gold Nanoparticles. Journal of the American Chemical Society, 133, 2120-2123.
- Patel, P.C., Hao, L., Au Yeung, W.S. and Mirkin, C.A. (2011) Duplex End Breathing Determines Serum Stability and Intracellular Potency of siRNA-Au NPs. Molecular Pharmaceutics, 8, 1285-1291.
- Ono, T., Miyawaki, S., Kimura, F., Kanamori, H., Ohtake, S., Kitamura, K., Fujita, H., Sugiura, I., Usuki, K., Emi, N., Tamaki, S., Aoyama, Y., Kaya, H., Naoe, T., Tadokoro, K., Yamaguchi, T., Ohno, R. and Ohnishi, K. (2011) BCR-ABL1 mutations in patients with imatinib-resistant Philadelphia chromosome-positive leukemia by use of the PCR-Invader assay. Leukemia Research, 35, 598-603.
- Jones, M.R., Macfarlane, R.J., Prigodich, A.E., Patel, P.C. and Mirkin, C.A. (2011) Nanoparticle Shape Anisotropy Dictates the Collective Behavior of Surface-Bound Ligands. Journal of the American Chemical Society, 133, 18865-18869.
- Blobel, J., Brath, U., Bernado, P., Diehl, C., Ballester, L., Sornosa, A., Akke, M. and Pons, M. (2011) Protein loop compaction and the origin of the effect of arginine and glutamic acid mixtures on solubility, stability and transient oligomerization of proteins. Eur Biophys J, 40, 1327-1338.
- Alicea, I., Marvin, J.S., Miklos, A.E., Ellington, A.D., Looger, L.L. and Schreiter, E.R. (2011) Structure of the Escherichia coli Phosphonate Binding Protein PhnD and Rationally Optimized Phosphonate Biosensors. Journal of Molecular Biology, 414, 356-369.
- Abian, O., Alfonso, P., Velazquez-Campoy, A., Giraldo, P., Pocovi, M. and Sancho, J. (2011) Therapeutic Strategies for Gaucher Disease: Miglustat (NB-DNJ) as a Pharmacological Chaperone for Glucocerebrosidase and the Different Thermostability of Velaglucerase Alfa and Imiglucerase. Molecular Pharmaceutics, 8, 2390-2397.
- Vedadi, M., Arrowsmith, C.H., Allali-Hassani, A., Senisterra, G. and Wasney, G.A. (2010) Biophysical characterization of recombinant proteins: A key to higher structural genomics success. Journal of Structural Biology, 172, 107-119.
- Serrano, I., Pelliccione, S. and Olmedilla, A. (2010) Programmed-cell-death hallmarks in incompatible pollen and papillar stigma cells of Olea europaea L. under free pollination. Plant Cell Rep, 29, 561-572.
- Laborde, S., Degrave, A., Lehmann, D., Jouette, S., Rofel, C., Muller, T., Hertzog, N., Rook, M. and Ribault, S. (2010) Detection of Mollicutes in bioreactor samples by real-time transcription-mediated amplification. Letters in Applied Microbiology, 50, 633-638.
- Yamamoto, M., Kakihana, K., Ohashi, K., Yamaguchi, T., Tadokoro, K., Akiyama, H. and Sakamaki, H. (2009) Serial monitoring of T315I BCR-ABL mutation by Invader assay combined with RT-PCR. Int J Hematol, 89, 482-488.
- Timofeevski, S.L., McTigue, M.A., Ryan, K., Cui, J., Zou, H.Y., Zhu, J.X., Chau, F., Alton, G., Karlicek, S., Christensen, J.G. and Murray, B.W. (2009) Enzymatic Characterization of c-Met Receptor Tyrosine Kinase Oncogenic Mutants and Kinetic Studies with Aminopyridine and Triazolopyrazine Inhibitors. Biochemistry, 48, 5339-5349.
- Seferos, D.S., Prigodich, A.E., Giljohann, D.A., Patel, P.C. and Mirkin, C.A. (2009) Polyvalent DNA Nanoparticle Conjugates Stabilize Nucleic Acids. Nano Letters
- Nano Lett., 9, 308-311.
- Ruschak, A.M. and Miranker, A.D. (2009) The Role of Prefibrillar Structures in the Assembly of a Peptide Amyloid. Journal of Molecular Biology, 393, 214-226.
- Min, T., Vedadi, M., Watson, D.C., Wasney, G.A., Munger, C., Cygler, M., Matte, A. and Young, N.M. (2009) Specificity of Campylobacter jejuni Adhesin PEB3 for Phosphates and Structural Differences among Its Ligand Complexes,. Biochemistry, 48, 3057-3067.
- Massich, M.D., Giljohann, D.A., Seferos, D.S., Ludlow, L.E., Horvath, C.M. and Mirkin, C.A. (2009) Regulating Immune Response Using Polyvalent Nucleic Acidâˆ’Gold Nanoparticle Conjugates. Molecular Pharmaceutics 6, 1934-1940.
- Manus, L.M., Mastarone, D.J., Waters, E.A., Zhang, X.-Q., Schultz-Sikma, E.A., MacRenaris, K.W., Ho, D. and Meade, T.J. (2009) Gd(III)-Nanodiamond Conjugates for MRI Contrast Enhancement. Nano Letters, 10, 484-489.
- Hurst, S.J., Hill, H.D., Macfarlane, R.J., Wu, J., Dravid, V.P. and Mirkin, C.A. (2009) Synthetically Programmable DNA Binding Domains in Aggregates of DNA-Functionalized Gold Nanoparticles. Small, 5, 2156-2161.
- Huang, X., Finerty Jr., P., Walker, J.R., Butler-Cole, C., Vedadi, M., Schapira, M., Parker, S.A., Turk, B.E., Thompson, D.A. and Dhe-Paganon, S. (2009) Structural insights into the inhibited states of the Mer receptor tyrosine kinase. Journal of Structural Biology, 165, 88-96.
- Giljohann, D.A., Seferos, D.S., Prigodich, A.E., Patel, P.C. and Mirkin, C.A. (2009) Gene Regulation with Polyvalent siRNAâˆ’Nanoparticle Conjugates. Journal of the American Chemical Society, 131, 2072-2073.
- Cremades, N., Velazquez-Campoy, A., Martinez-Julvez, M., Neira, J.L., Perez-Dorado, I., Hermoso, J., Jimenez, P., Lanas, A., Hoffman, P.S. and Sancho, J. (2009) Discovery of Specific Flavodoxin Inhibitors as Potential Therapeutic Agents against Helicobacter pylori Infection. ACS Chemical Biology 4, 928-938.
- Shaya, D., Hahn, B.-S., Park, N.Y., Sim, J.-S., Kim, Y.S. and Cygler, M. (2008) Characterization of Chondroitin Sulfate Lyase ABC from Bacteroides thetaiotaomicron WAL2926. Biochemistry, 47, 6650-6661.
- Millstone, J.E., Georganopoulou, D.G., Xu, X., Wei, W., Li, S. and Mirkin, C.A. (2008) DNA-Gold Triangular Nanoprism Conjugates. Small, 4, 2176-2180.
- Altieri, S.L., Clayton, G.M., Silverman, W.R., Olivares, A.O., De La Cruz, E.M., Thomas, L.R. and Morais-Cabral, J.o.H. (2008) Structural and Energetic Analysis of Activation by a Cyclic Nucleotide Binding Domain. Journal of Molecular Biology, 381, 655-669.
- Stevens, R.C., Sancho, J. and Martinez, A. (2007) Rescue of misfolded proteins and stabilization by small molecules. Methods Mol Biol, 648, 313-324.
- Seferos, D.S., Giljohann, D.A., Hill, H.D., Prigodich, A.E. and Mirkin, C.A. (2007) Nano-Flares: Probes for Transfection and mRNA Detection in Living Cells. Journal of the American Chemical Society, 129, 15477-15479.
- Davis, T.L., Walker, J.R., Finerty Jr, P.J., Mackenzie, F., Newman, E.M. and Dhe-Paganon, S. (2007) The Crystal Structures of Human Calpains 1 and 9 Imply Diverse Mechanisms of Action and Auto-inhibition. Journal of Molecular Biology, 366, 216-229.
- Bakalova, R., Zhelev, Z., Kubo, T., Mileva, M. and Ohba, H. (2007) Dual-labeled telomere sensing probes for quantification of telomerase activity assay. Journal of Biochemical and Biophysical Methods, 70, 503-506.
- Yamamoto, G., Irie, T., Aida, T., Nagoshi, Y., Tsuchiya, R. and Tachikawa, T. (2006) Correlation of invasion and metastasis of cancer cells, and expression of the RAD21 gene in oral squamous cell carcinoma. Virchows Arch, 448, 435-441.
- Knight, J.D., Hebda, J.A. and Miranker, A.D. (2006) Conserved and Cooperative Assembly of Membrane-Bound Î±-Helical States of Islet Amyloid Polypeptide. Biochemistry, 45, 9496-9508.
- Bullock, A.N., Debreczeni, J.Ã., Fedorov, O.Y., Nelson, A., Marsden, B.D. and Knapp, S. (2005) Structural Basis of Inhibitor Specificity of the Human Protooncogene Proviral Insertion Site in Moloney Murine Leukemia Virus (PIM-1) Kinase. Journal of Medicinal Chemistry 48, 7604-7614.
- Knight, J.D. and Miranker, A.D. (2004) Phospholipid Catalysis of Diabetic Amyloid Assembly. Journal of Molecular Biology, 341, 1175-1187.
- Abbott labs
- Amgen Inc.
- Astex Technology Ltd
- Boehringer Ingelheim Pharma GmbH & Co.
- Gen-Probe Incorporated
- Glaxo SmithKline
- Hoffmann-La Roche Inc.
- Merck & Co., Inc.
- Millipore Corporation
- MLT Research Ltd
- Pfizer Global research & Development
- Sanofi Aventis
- Sensor technologies Inc.
- USB Corporation
- Duke University
- National Institutes of Health (NIH / NIEHS)
- National Research Council of Canada
- Sandia National Laboratories
- Southern Illinois University
- Northwestern University
- Oxford University
- Trinity University
- University of California Berkeley
- University of California San Diego
- University of Georgia
- University of Illinois at Chicago
- University of Texas
- University of Toronto
- Vanderbilt University
- Yale University
- Toronto General Hospital
- V. A. Medical Center
- And many more…